Collected reprints: Abstract 2708

Collected reprints

Abstract

Chen, H.-Y.; Leu, Y.-T.; Roelants, I. (1992). Quantification of arginine requirements of juvenile marine shrimp, Penaeus monodon, using microencapsulated arginine. Mar. Biol. 114: 229-233

Using microencapsulated L-arginine, the quantitative requirement for amino acids has been determined for the first time for a shrimp species, which can not effectively utilize crystalline amino acids. In an 8 wk feeding trial (1990), juvenile Penaeus monodon were fed casein based purified diets containing one of six levels (13.1, 17.7, 22.3, 26.9, 31.5 and 36.1 g/kg diet) of arginine. In addition to the protein-bound arginine already present in the casein of the test diets, pure arginine was supplemented by L-arginine microencapsulated in cellulose acetate phthalate, which is easily assimilated. The arginine level required for optimal growth was determined by broken-line model analysis of weight gain data to 25.0 g/kg diet (= 54.7 g/kg protein). Examination of the hemolymph 3h after feeding revealed that the free arginine level in the hemolymph had not increased as a result of increasing levels of the dietary arginine. There was an abrupt increase of urea in the hemolymph when the arginine requirement of the shrimp had been met.

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