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Nitrogen incorporation and retention by bacteria, algae and fauna in subtropical, intertidal sediment: an in situ 15N labelling study [Stikstof-incorporatie en -retentie door bacteriën, algen en fauna in subtropisch, intertidaal sediment]
Citation
Veuger B., Soetaert K. 2006: Nitrogen incorporation and retention by bacteria, algae and fauna in subtropical, intertidal sediment: an in situ 15N labelling study. Netherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at http://data.nioo.knaw.nl/imis.php?module=dataset&dasid=1228

Archived data
Availability: Unrestricted
The data are freely available to anybody and may be used for any purpose. Usage acknowledgement may be required

Description
This database contains the results of an in situ 15N labelling study in intertidal, subtropical sediment of the Brunswick estuary in Australia. more

We performed a 15N-labeling study to investigate nitrogen incorporation and retention by the benthic microbial community (bacteria and benthic microalgae), and fauna in the intertidal sediment of the subtropical, Australian Brunswick Estuary. The main experiment involved an in situ 15N pulse chase experiment. After injection of 15NH4 into the sediment, 15N was traced into bulk sediment, total hydrolysable amino acids (THAAs, representing bulk proteinaceous biomass), the bacterial biomarker D-alanine, and fauna over a 30-day period. Additional experiments included short-term (24 h) incubations of sediment cores injected with different 15Nlabeled substrates (NH4 +, NO3 -, urea and an amino acid mixture) and sediment core incubations for analysis of benthic fluxes of O2, dissolved inorganic carbon (DIC), NH4 +, Nox -, dissolved organic nitrogen (DON), and N2. 15N was rapidly incorporated and strongly retained in microbial biomass (THAAs) during the 30-day period in situ, indicating efficient recycling of 15N by the benthic microbial community. Analysis of 15N in D-alanine revealed a major bacterial contribution (50-100%) to total microbial 15N incorporation and retention. 15N was also incorporated into fauna via grazing on 15N-labeled microbial biomass, but this was a negligible fraction (< 1%) of total 15N in the sediment. Altogether, results show that efficient recycling of nitrogen by the benthic microbial community can be an important mechanism for nitrogen retention in the sediment and an important pathway supporting benthic microbial production.

Scope
Themes:
Biology, Biology > Benthos, Biology > Flora, Bottom samples (e.g. core, dredge, grab), Water composition, Water composition > Nutrients
Keywords:
Brackish water, Terrestrial, Amino acids, Bacteria, Benthos, Isotopic labelling, Nitrogen isotopes, Sediments, PSE, Australia, New South Wales

Geographical coverage
PSE, Australia, New South Wales Stations [Marine Regions]
Brunswick estuary

Temporal coverage
2006

Parameters
Alkalinity, Ammonia (NH3), Average concentration of total hydrolysable amino acids (THAA), Biotic isotopic signature 15N (delta), C:N ratio (molair), Chlorophyll a, D-Alanine, Dissolved oxygen (O2), Isotope Nitrogen (N), Nitrate (NO3-) + Nitrite (NO2-), Organic carbon (OC), pH, Total dissolved nitrogen (TDN)

Contributor
Koninklijk Nederlands Instituut voor Onderzoek der Zee; NIOZ Yerseke, moredata owner

Dataset status: Completed
Data type: Data
Data origin: Research: field experiment
Metadatarecord created: 2007-04-18
Information last updated: 2009-10-08
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