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Nannochloropsis mass cultures
Kromkamp J. T., Stal L. 2008: Short term variation in photosynthetic parameters of Nannochloropsis grown in two different types of outdoor mass cultures. Netherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at

Archived data
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Notes: Release with permission of the appropriate parties

Data describes photosynthetic behaviour of Nannochloropsis in a high rate algal pond and a flat panel photobioreactor more

Nannochloropsis sp. (Eustigmatophyta) is a unicellular algal species popular for mass algal cultures because it contains high concentrations of the polyunsaturated fatty acid eicosapentaenoic acid (EPA- 20:5É3). In this experiment we followed the photosynthetic behaviour and biomass parameters of this alga during 50h in two outdoor mass cultures: a flat panel photobioreactor (FPP) and a high rate algal pond (HRAP) in order to detect acclimation patterns to different optical conditions, with the FPP having a small gradient in light intensity and the HRAP having large gradient in irradiance. Algae of both cultures showed a depression of photosynthetic activity during the afternoon, which was more pronounced in the FPP. Despite the fact that the maximum irradiance experienced by the algae was the same in both systems, the HRAP algae showed classic shade adaptation whereas the FPP algae showed high light acclimation in photosynthetic parameters. The FPP showed diurnal changes in the maximum rate of photosynthesis and respiration and maximum rates of photosynthesis were lower at night than during the day. High temperatures (41 oC) on day 3 in the FPP caused a large reduction in Fv/Fm and an over-reduction of the PSII acceptors which did not recover during the light period. PAM measurements with and without dark adaptation showed that this decreased photosynthetic activity was due to both chronic and dynamic downregulation. Photodamage was higher in the FPP, but most damaged seem to be repaired during the evening. A comparison between the quantum efficiencies for PSII charge separation and oxygen evolution revealed a close coupling between the two.

Biology > Pigments (e.g. chlorophyll), Biology > Plankton, Biology > Productivity - biomass, Fisheries > Aquaculture, Physical > Optical measurements
Marine, Mass culture, Photosynthesis, Pulse amplitude modulation (PAM), Quantum efficiency, Israel, Eilat, Eustigmatophyceae, Nannochloropsis D.J.Hibberd, 1981

Geographical coverage
Israel, Eilat [Marine Regions]

Temporal coverage
1 April 2008 - 3 April 2008

Taxonomic coverage
Eustigmatophyceae [WoRMS]
Nannochloropsis D.J.Hibberd, 1981 [WoRMS]

Chlorophyll a, Fluorescent induction (OIJP transient) Methodology , Irradiance Methodology , Oxygen (O) evolution Methodology , pH, Photosystem II complex (PSII) efficiency Methodology , Photosystem II complex (PSII) electron transport (ETR), Temperature, Total carotenoids Methodology
Fluorescent induction (OIJP transient): Fluorescent induction (OIJP transient) detection
Instrument used Fluorescence induction curves: A portable fluorometer, the Aquapen AP 100 (Photon System Instruments), fitted with red light-emitting diodes (LEDs) to provide saturating light intensity was used to follow the polyphasic rise of chl a fluorescence in both outdoor cultures, the so-called Kautsky curve.
Irradiance: Irradiance measurement
Irradiance: Irradiance measurement
Oxygen (O) evolution: Detection of changes in oxygen evolution with a Light Pipette
According to Dubinsky et al (1987). This instrument consists of a light source connected to a cuvette with a micro-oxygen electrode, a quantum sensor, a temperature-controlled water bath and a computer. It enables sensitive detection of changes in oxygen evolution/consumption and an easy collection and digitalisation of data.
Photosystem II complex (PSII) efficiency: Photosystem II complex (PSII) efficiency measurements
Following instruments were used: Flow-through water PAM: This is a highly sensitive chlorophyll fluorometer designed for continuously monitoring photosynthetic activity of phytoplankton and algal suspensions. Dual pulse amplitude modulation (PAM): The dual PAM fluorometer (Walz) measures the Photosystem II (PSII) efficiency simultaneously with the absorption changes of Photosystem I (PSI) using the pulse modulation principle in the dual wavelength approach (Klughammer & Schreiber 2008).
Total carotenoids: Methanol extraction
According to Lichtentaler (1987)

Koninklijk Nederlands Instituut voor Onderzoek der Zee; NIOZ Yerseke, moredata owner

Based on this dataset
Sukenik, A. et al. (2009). Photosynthetic performance of outdoor Nannochloropsis mass cultures under a wide range of environmental conditions. Aquat. Microb. Ecol. 56(2-3): 297-308., more
Kromkamp, J.C. et al. (2009). Short-term variations in photosynthetic parameters of Nannochloropsis cultures grown in two types of outdoor mass cultivation systems. Aquat. Microb. Ecol. 56(2-3): 309-322., more

Dataset status: In Progress
Data type: Data
Data origin: Research
Metadatarecord created: 2009-10-21
Information last updated: 2009-11-18
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