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DIPP: Dual Isotope Paulina Polder
Citation
Veuger and Van Oevelen, 2156: Data from Dual Isotope labeling at Paulina Polder. etherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at http://data.nioo.knaw.nl/imis.php?module=dataset&dasid=2156

Archived data

Archived file:
Availability: Restricted
The data are withheld from general circulation and disclosure but access may be obtained on a case-by-case basis through negotiation

Description
Available data from the experiment in which sediment was injected with 13C-glucose and 15N-ammonium. Experiment was conducted 1) in situ, 2) sediment cores and 3) slurry bottles more

13C-glucose and 15N-ammonium was injected in three ways:
1) in situ sediments, 4 week incubation
2) retrieved sediment cores, 4 week incubation
3) slurry bottles, one year incubation
The different treatments were sampled for:
  • 13C/15N incorporation in POM
  • 13C incorporation in PLFA
  • 13C incorporation in FA
  • 13C incorporation in sugars
  • 13C/15N incorporation in Hydrolyzable amino acids
  • 13C/15N incorporation in meio- and macrofauna
  • pigment composition
  • three macrofaunal species were analyzed for 13C/15N in FA and HAAs


Scope
Themes:
Biology, Biology > Benthos, Biology > Invertebrates, Biology > Organic (& bio-) chemistry, Bottom samples (e.g. core, dredge, grab)
Keywords:
Marine, Bacteria, Benthic infauna, Carbon isotopes, Macrobenthos, Meiobenthos, Nauplii, Nitrogen isotopes, Pigments, Radioactive labelling, Sediments, ANE, Netherlands, Westerschelde, PaulinaSchor, Arenicola marina, Arenicola marina (Linnaeus, 1758), Bivalvia, Brachyura, Cerastoderma edule (Linnaeus, 1758), Copepoda, Eteone Savigny, 1818, Foraminifera, Glycera, Heteromastus filiformis (Claparède, 1864), Macoma balthica, Nematoda, Nereis diversicolor, Oligochaeta, Ostracoda, Polychaeta, Pygospio elegans Claparède, 1863, Scrobicularia plana (Da Costa, 1778), Tharyx marioni (de St Joseph)

Geographical coverage
ANE, Netherlands, Westerschelde, PaulinaSchor [Marine Regions]

Temporal coverage
14 June 2006 - 20 February 2007

Taxonomic coverage
Arenicola marina (Linnaeus, 1758) [WoRMS]
Arenicola marina (Linnaeus, 1758) [WoRMS]
Bacteria [WoRMS]
Bivalvia [WoRMS]
Brachyura [WoRMS]
Cerastoderma edule (Linnaeus, 1758) [WoRMS]
Copepoda [WoRMS]
Eteone Savigny, 1818 [WoRMS]
Foraminifera [WoRMS]
Glycera Lamarck, 1818 [WoRMS]
Heteromastus filiformis (Claparède, 1864) [WoRMS]
Macoma balthica (Linnaeus, 1758) [WoRMS]
Nematoda [WoRMS]
Nereis (Hediste) diversicolor O.F. Müller, 1776 [WoRMS]
Oligochaeta [WoRMS]
Ostracoda [WoRMS]
Polychaeta [WoRMS]
Pygospio elegans Claparède, 1863 [WoRMS]
Scrobicularia plana (da Costa, 1778) [WoRMS]
Tharyx marioni (Saint-Joseph, 1894) [WoRMS]

Parameters
Amino acids Methodology , C:N ratio Methodology , Carotene Methodology , Chlorophyll a Methodology , Chlorophyll c Methodology , Diatoxanthin Methodology , Fucoxanthin Methodology , Isotope Carbon (C), Isotope Nitrogen (N), Organic carbon (OC) Methodology , Organic nitrogen (N) Methodology , Phaeophytin Methodology , Phospholipid-derived fatty acids (PLFAs) analysis Methodology , Pigment analysis Methodology
Amino acids: Amino acids in sediment measurements
As described by: Veuger, B. et al. (2005). Analysis of 15N incorporation into D-alanine: a new method for tracing nitrogen uptake by bacteria. Limnol. Oceanogr: Methods 3: 230-240.

Amino acids: Amino acids in sediment measurements
As described by: Veuger, B. et al. (2005). Analysis of 15N incorporation into D-alanine: a new method for tracing nitrogen uptake by bacteria. Limnol. Oceanogr: Methods 3: 230-240.
C:N ratio: EA-IRMS
Carotene: HPLC
Chlorophyll a: HPLC
Chlorophyll c : HPLC
Diatoxanthin: HPLC
Fucoxanthin: HPLC
Organic carbon (OC): EA-IRMS
Organic nitrogen (N): EA-IRMS
Phaeophytin: HPLC
Phospholipid-derived fatty acids (PLFAs) analysis: Phospholipid fatty acid analysis (PLFA) by GC-c-IRMS methodology
Methodology is based on: Boschker, H. T. S. 2004. Linking microbial community struc-ture and functioning: Stable isotope (13C) labeling in com-bination with PLFA analysis, p. 1673-1688. In G. A.Kowalchuk, d. B. F.J., I. M. Head, A. D. Akkermans, and J. D.van Elsas [eds.], Mol.

Phospholipid-derived fatty acids (PLFAs) analysis: Phospholipid fatty acid analysis (PLFA) methodology
Pigment analysis: HPLC

Contributor
Royal Netherlands Academy of Arts and Sciences; Netherlands Institute of Ecology (NIOO-KNAW), moredata owner

Dataset status: In Progress
Data type: Data
Data origin: Data collection
Metadatarecord created: 2009-10-21
Information last updated: 2013-09-10
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