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Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary [Metabolisme, reproductie en vervelling als eindpunten voor het bestuderen van endocriene verstoring bij Neomysis integer in het Schelde-estuarium]
Citation
Ghekiere A., Verslycke T., De Smet L., Van Beeumen J., Janssen C.R.; Environmental toxicology research group - Ugent; (2004): Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary

Access data
Archived data
Dedicated website
Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
This dataset results out of the study into ecotoxicological evaluation of the effects of endocrine disrupting substances occurring in the Scheldt estuary on resident mysid shrimp populations (laboratory and field studies). more

The data results from a number of lab tests.To evaluate the possible effects of the pollutants on mysid shrimp, acute and chronic test will be performed in the laboratory. Different endpoints to study endocrine disruption are metabolism, reproduction, vitellogenesis and moulting.

Scope
Themes:
Biology, Biology > Invertebrates, Environmental quality/pollution
Keywords:
Biomarkers, Crustacea, Energy, Estrogens, Metabolism, Moulting, Reproduction, Vitellogenesis, ANE, Scheldt Estuary, ANE, Netherlands, Westerschelde, Belgium, Zeeschelde, ANE, Netherlands, Westerschelde, Bath, ANE, Netherlands, Westerschelde, Hansweert, ANE, Netherlands, Westerschelde, Terneuzen, ANE, Netherlands, Westerschelde, Verdronken Land van Saeftinge, ANE, Netherlands, Westerschelde, Vlissingen, Belgium, Zeeschelde, Antwerp, Belgium, Zeeschelde, Doel, Neomysis integer (Leach, 1814)

Geographical coverage
ANE, Scheldt Estuary [Marine Regions]
ANE, Netherlands, Westerschelde [Marine Regions]
Belgium, Zeeschelde [Marine Regions]
ANE, Netherlands, Westerschelde, Bath Stations [Marine Regions]
S12
Coordinates: Long: 4,225; Lat: 51,365 [WGS84]
ANE, Netherlands, Westerschelde, Hansweert Stations [Marine Regions]
S07
Coordinates: Long: 4; Lat: 51,4367 [WGS84]
ANE, Netherlands, Westerschelde, Terneuzen Stations [Marine Regions]
S04
Coordinates: Long: 3,825; Lat: 51,345 [WGS84]
ANE, Netherlands, Westerschelde, Verdronken Land van Saeftinge Stations [Marine Regions]
S09
Coordinates: Long: 4,0783; Lat: 51,37 [WGS84]
ANE, Netherlands, Westerschelde, Vlissingen Stations [Marine Regions]
S01
Coordinates: Long: 3,57; Lat: 51,4167 [WGS84]
Belgium, Zeeschelde, Antwerp Stations [Marine Regions]
S22
Coordinates: Long: 4,3917; Lat: 51,2188 [WGS84]
Belgium, Zeeschelde, Doel Stations [Marine Regions]
S15
Coordinates: Long: 4,2733; Lat: 51,3133 [WGS84]

Temporal coverage
4 December 2002 - 6 December 2002
17 March 2003 - 21 March 2003
30 June 2003 - 4 July 2003
4 February 2004 - 5 February 2004

Taxonomic coverage
Neomysis integer (Leach, 1814) [WoRMS]

Parameters
Growth factor Methodology , Growth rate Methodology , Mysid energy metabolism Methodology , Mysid intermoult period Methodology , Mysid testosteron metabolism Methodology , Mysid vitelline concentration Methodology
Growth factor: Moulting measuring protocol growth factor
and the increase in length at each molting event (the growth factor, GF in % of the pre-molt length)
Growth rate: Moulting measuring protocol growth rate
length incresase (growth rate, µm/day) during IMPs
Mysid energy metabolism: Cellular Energy Allocation
The energy allocation of the estuarine mysid Neomysis integer were investigated using the cellular energy allocation assay (CEA), energy reserves (protein, lipid and sugar) and energy consumption (as derived from the cellular respiration rate) were integrated into a general indicator of physiological stress. Adult Neomysis integer specimens were sorted out on board, sex was not taken into account and no selection was made to avoid gravid females. The collected mysids were shock-frozen in liqui
Mysid intermoult period: Moulting measuring protocol intermoult period
time (days) between two successive molts (intermolt period; IMP)
Mysid testosteron metabolism: LCMS testosteron
In vivo exposure to testosterone: Juvenile Neomysis integer were taken from the different samplingsites during the campaigns of March and June and individually placed into 5 ml glass recipients containing 2 ml water (5 PSU). Two micrograms of testosterone dissolved in 10 µl methanol was added to the recipient and a 6 h exposure period was applied. Extraction and LC-MSn analysis of testosterone metabolites and endogenous steroids: N. integer were isolated from the exposure medium, shock-
Mysid vitelline concentration: Enzyme-linked immunosorbent assay (ELISA)
-Vitellin purification (gel filtration) -polyclonal anitbody production against vitellin -development of ELISA protocol

Contributors
Belgian Science Policy (BELSPO), moredata owner
Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Toegepaste ecologie en milieubiologie; Laboratorium voor Milieutoxicologie en aquatische ecologie; Onderzoeksgroep voor Milieutoxicologie (ECOTOX), moredata ownerdata creatordata manager

Related datasets
Parent dataset:
ENDIS-RISKS fysico-chemical measurements and hyperbenthos in the Scheldt river, 2002-2006., more
Published in:
EurOBIS: European Ocean Biogeographic Information System, more
IMERS: Integrated Marine Environmental Samples and Readings, more

Project
ENDIS-RISKS: Endocrine disruption in the Scheldt Estuary: distribution, exposure and effects, more

Publication
Based on this dataset:
Ghekiere, A. et al. (2004). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea). Comp. Biochem. Physiol. 138(4): 427-433. dx.doi.org/10.1016/j.cbpb.2004.05.012, more
Ghekiere, A. et al. (2006). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea), in: Ghekiere, A. Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). pp. 24-35, more

Dataset status: Completed
Data type: Data
Data origin: Research
Metadatarecord created: 2004-05-10
Information last updated: 2017-11-17
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