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Antarctic polar desert surface soil microbiome
Citation
Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B (2019): Antarctic polar desert surface soil microbiome. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome&v=1.0 https://dx.doi.org/10.15468/pematf

Access data
Archived data
Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica). more

Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis.
Study Extent: Samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005.
Method step description:
  1. Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA).
  2. Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland).

Scope
Keywords:
Metadata, Sequencing, Antarctica, Archaea, Bacteria, Viruses

Geographical coverage
Antarctica Stations [Marine Regions]
Robinson Ridge

Temporal coverage
December 2005

Taxonomic coverage
Archaea [WoRMS]
Bacteria [WoRMS]
Viruses [WoRMS]

Parameter
Molecular data

Contributors
Australian Antarctic Division (AAD), moredata creator
Australian Centre for Astrobiology (ACA), moredata creator
GNS Science; Wairakei Research Centre, moredata creator
Monash University, moredata creator
The University of Queensland (UQ), moredata creator

Dataset status: Completed
Data type: Metadata
Data origin: Research: field survey
Metadatarecord created: 2019-04-11
Information last updated: 2019-04-11
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy