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Insights from hybrid zones: testing for habitat choice, divergent selection and genomic architecture in Littorina saxatilis adaptive divergence
Citation
Raffini, F.; Butlin, R.; Department of Animal and Plant Sciences. University of Sheffield: United Kingdom; (2019): Insights from hybrid zones: testing for habitat choice, divergent selection and genomic architecture in Littorina saxatilis adaptive divergence. http://www.assembleplus.eu/information-system?module=dataset&dasid=6371

Availability: Unrestricted after moratorium period
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Description
Dataset describing position, habitat and date of collection, snail phenotype-genotype data, and positions and habitats at movement observation and recapture seks and developmental stage data. more

48 snails were collected in the field (Centinela, Baiona, Spain; coordinates: N 42° 4' 38.06", W 8° 53' 47.47") in the wave, crab and hybrid zone respectively (total sample size: 144). Prior to collection, the habitat and position of each individual was recorded with a survey instrument (total station) to <10cm resolution. Specimens were transported to the lab where they were marked with individual waterproof labels and non-toxic nail polish along the margin of the aperture (to track growth); shell thickness was measured with a gauge; shells photographed for morphology; and DNA collected from mucus (non-destructive DNA sampling protocol using TFA cards). Individuals were then released in the field: each collection group (crab/hybrid/wave) was randomly split in three subgroups and released in the hybrid, crab and wave habitats, respectively, recording the precise release position and habitat characteristics. One day later, the positions and habitats of marked snails was recorded for movement. This round of collection-mark-release was repeated four times (one release included only 48 snails from the hybrid zone due to not permissive weather), for a final number of 480 released snails. One month later, in June, surviving snails were recaptured, with positions and habitats recorded, phenotyped (growth, weight) and dissected for sex determination and tissue collection for additional DNA analysis.

Scope
Themes:
Biology > Benthos
Keywords:
Marine, Adaptation, divergent selection, genomic architecture, Habitat choice, Hybrid zones, Parallel evolution, Reproductive isolation, Speciation, Spain, Vigo, Littorina saxatilis (Olivi, 1792)

Geographical coverage
Spain, Vigo

Temporal coverage
23 March 2019 - 11 April 2019
1 June 2019 - 10 June 2019

Taxonomic coverage
Littorina saxatilis (Olivi, 1792) [WoRMS]

Parameters
Body size, Developmental stages, Genotype, Growth, Habitat, Sex, Weight

Contributors
University of Sheffield; School of Biological Sciences; Department of Animal and Plant Sciences (APS), moredata creator

Related datasets
Other relation:
Illuminating adaptive divergence through hybrid zones in Littorina saxatilis, more

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, more

Dataset status: Completed
Data type: Data
Data origin: Research: field experiment
Metadatarecord created: 2019-12-03
Information last updated: 2020-04-16
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy