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Toxicity of cryoprotectants on early developmental stages of blue mussel, Mytilus galloprovincialis
Citation
Heres, P.; Rodriguez-Riveiro, R.; Troncoso, J.; Paredes, E.; Grupo de Investigación en Ecología Costera: Uvigo-EcoCost: Spain; (2021): Toxicity of cryoprotectants on early developmental stages of blue mussel, Mytilus galloprovincialis. Marine Data Archive.

Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
Experimental results of toxicity tests of cryoprotecting agents for Blue mussel, Mytilus galloprovincialis, on early developmental stages. more

Mature individuals deposited in PVC tanks with Filtered Sea Water (FSW, 35-37 promille). Cyclic changes of water temperature, from 14°C to 20°C to induce spawning. Spawning individuals put into separate plastic containers to collect sperm and oocytes. After fertilization, incubation of fertilized eggs at a density of 40 individuals/mL. Periodical sampling to collect fertilized egg, trochophore larval stage (18-20h post-fertilization) and 48 and 72h-old D-larval stage for experiments. Cryoprotecting agent solutions prepared at twice the final concentration for the experiments. For toxicity tests, preparation of increasing concentrations (from 1 to 6 M) of Ethylene-Glycol (EG), Propylene-Glycol (PG), Dimethyl-Sulfoxide (Me2SO) and Glycerol (GLY) prepared in FSW. For cryopreservation experiments, 10% (v/v) EG + 0.4M (w/v) trehalose (TRE). Three replicates were assayed for each CPA concentration and control trials. Cryoprotectant solutions added 1:1 to a FSW mL with 400-600 clam individuals in one step. 15 minutes of exposure at room temperature (18-20°C). Samples diluted with FSW 1:1. Then, incubation in 20 mL of clean FSW at 18°C until D-larval stage was reached for exposed fertilized eggs and trochophores, 48h of incubation in the case of D-larval stage. After that, cells fixed with formalin for normal and abnormal D-larval counts. Obtention of normal and abnormal larval precentages, then arcsin-square transformation of data. For toxicity results, statistical analysis following ANOVA-one-way and calculations of NOEC and LOEC levels by post-hoc Dunnet test. For cryopreservation experiments, Bonferroni post-hoc test, assuming homogeneity of variances.

Scope
Themes:
Biology > Invertebrates
Keywords:
Marine, Cryopreservation, Cryoprotecting agents, Experimental research, Larval development, Marine invertebrates, Marine molluscs, Toxicants, Mytilus galloprovincialis Lamarck, 1819

Temporal coverage
11 October 2017 - 13 October 2017

Taxonomic coverage
Mytilus galloprovincialis Lamarck, 1819 [WoRMS]

Parameter
Count of larvae

Contributors
University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera (EcoCost), moredata creator

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, more

Publication
Based on this dataset
Heres, P. et al. (2019). Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages. Cryobiology 86: 40-46. https://hdl.handle.net/10.1016/j.cryobiol.2019.01.001, more

Dataset status: Completed
Data type: Data
Data origin: Research: lab experiment
Metadatarecord created: 2021-04-29
Information last updated: 2021-10-18
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