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Temporal patterns of bacterial diversity in a changing environment [Temporele patronen van bacteriële diversiteit in een veranderende omgeving]

Archived data
Availability: Restricted
The data are withheld from general circulation and disclosure but access may be obtained on a case-by-case basis through negotiation

Notes: Restrictions available at contact person

Description
The objective of this dataset was to evaluate possible changes in bacterial communities in relation to changing food availability. more

This dataset is the result of DNA extraction, PCR amplification of 16S rDNA and DGGE (Denaturing Gradient Gel Electrophoresis) analysis of cores taken at BCS stations 115 and 330 during spring and autumn of 2003. The objective of this dataset was to evaluate possible changes in bacterial communities in relation to changing food availability.

Scope
Themes:
Biology, Biology > Benthos, Biology > Ecology - biodiversity
Keywords:
Marine, Bacteria, Biodiversity, ANE, Belgium, Belgian Continental Shelf (BCS), ANE, Belgium, West Coast, Westdiep, ANE, Belgium, Zeeland Banks, Goote Bank

Geographical coverage
ANE, Belgium, Belgian Continental Shelf (BCS) [Marine Regions]
ANE, Belgium, West Coast, Westdiep Stations [Marine Regions]
Station 115bis
Coordinates: Long: 2,6183; Lat: 51,1517 [WGS84]
ANE, Belgium, Zeeland Banks, Goote Bank Stations [Marine Regions]
Station 330
Coordinates: Long: 2,8083; Lat: 51,4333 [WGS84]

Temporal coverage
October 2002 - October 2003
Monthly

Taxonomic coverage
Bacteria [WoRMS]

Parameter
Bacterial diversity Methodology
Bacterial diversity: Determination of bacterial diversity
Samples The cores for the study of variation in the biodiversity of the bacterial community were taken to the laboratory closed, preserving that way the overlaying water. In a bench flow cores with were taken in each sampling site, using a piston-style core with a 3.57 cm inner diameter. The top first centimetre of sediment was taken and split into two 2ml ependorfs for further DNA extraction. This was replicated twice, obtaining so 4 sub-samples from each of the three replicated cores taken at

Contributors
Belgian Science Policy (BELSPO), moredata owner
Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Mariene Biologie (MARBIOL), moredata ownerdata creatordata manager

Related datasets
(Partly) included in:
PROJECT DATA: Integrated data from projects PODOI and Trophos (1997-2006), more

Project
TROPHOS: Higher trophic levels in the Southern North Sea, more

Publication
Based on this dataset:
Franco, M.A.; Vanaverbeke, J.; Steyaert, M.; Vincx, M. (2004). Dynamics of meiobenthos and bacteria at two contrasting sites at the Belgian Continental Shelf, in: Hogere trofische niveaus in de Zuidelijke Noordzee 'TROPHOS': wetenschappelijk verslag voor de periode van 01/02/2003-31/01/2004. pp. 1-8, more

Dataset status: Completed
Data type: Data
Data origin: Research
Metadatarecord created: 2004-05-10
Information last updated: 2015-10-27
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