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Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)
Citation
Luo W, Ding H, Li H, Ji Z, Huang K, Zhao W, Yu Y, Zeng Y (2021): Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_communities_colored_snow_antarctica_2017&v=1.0 https://doi.org/10.15468/u6zfw7
Contact: Luo, Wei

Access data
Archived data
Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Notes: The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Description
Amplicon sequencing dataset (Illumina MiSeq) targeting Eukaryota (18S ssu rRNA) and Bacteria (16S ssu rRNA) in red or green colored snow samples (n=10) from Fildes Peninsula, Antarctica (2017). more

Samples were taken aseptically, using a 30 × 30 cm colored snow squares for each sampling station.
Colored snow samples (500 mL) from Fildes Peninsula (Antarctica) taken on 2017-01-30.
Samples were allowed to naturally melt (≤ 10 °C) and were subsequently filtered through a 0.2 μm nucleopore membrane filter (Whatman). The filters were frozen at − 80 °C in cetyltrimethyl ammonium bromide (CTAB) buffer until analysis. DNA extraction was performed as described by Luo et al. (2017). PCR of microbial eukaryotes targeted the V4 region of the 18S ribosomal RNA (rRNA) gene: forward primer 3NDf (5ʹ-GGCAAGTCTGGTGCCAG-3ʹ) and reverse primer V4_euk_R2 (5ʹ-ACGGTATCTRATCRTCTTCG-3ʹ). The V4–V5 hypervariable regions of the bacte- rial 16S rRNA gene were amplified using PCR with the universal primers 515F-Y (5ʹ-GTGYCAGCMGCCGCG GTAA-3ʹ) and 926R (5ʹ-CCGYCAATTYMTTTRAG TTT-3ʹ).
PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany), and quality was examined with a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations and sequenced on an Illumina MiSeq2000 platform.

Scope
Themes:
Biology > Ecology - biodiversity
Keywords:
Fresh water, Terrestrial, Eukaryotes, Metadata, Microbial activities, Rrna, Sequencing, Snow, Antarctica, Bacteria

Geographical coverage
Antarctica Stations [Marine Regions]
Fildes Peninsula

Temporal coverage
30 January 2017

Taxonomic coverage
Bacteria [WoRMS]

Parameters
16S, 18S, Presence of biota

Contributors
Polar Research Institute of China (PRIC), moredata creator

Related datasets
Published in:
AntOBIS: Antarctic Ocean Biodiversity Information System, more
(Partly) included in:
RAS: Register of Antarctic Species, more

Dataset status: Completed
Data type: Metadata
Data origin: Research: field survey
Metadatarecord created: 2021-07-06
Information last updated: 2021-07-06
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