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RGB-statistics derived from Nile red-stained reference plastics for the construction of the PIM (Polymer Identification Model)
Citable as data publication
Meyers, N.; Catarino, A.I.; Declercq, A.M.; Brenan, A.; Devriese, L.; Vandegehuchte, M.; De Witte, B.; Janssen, C.; Everaert, G.; Flanders Marine Institute (VLIZ); Flanders Research Institute for Agriculture, Fisheries and Food (ILVO); Ghent University Laboratory for Environmental Toxicology (GhEnToxLab): Belgium; (2021): RGB-statistics derived from Nile red-stained reference plastics for the construction of the PIM (Polymer Identification Model). Marine Data Archive. https://doi.org/10.14284/511
Contact: Meyers, Nelle

Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
Dataset containing RGB-statistics extracted from photographed fluorescent reference plastics stained with Nile red. The most abundantly produced plastic polymers worldwide considered for this dataset. The spectral data was used to construct a supervised machine learning model that allows to accurately identify the polymer types microplastics belong to, in a cost- and time-efficient way. more

The dataset was built to train and validate the ‘Polymer Identification Model’ (PIM) in R and contains Red, Green and Blue (RGB) statistics extracted from Nile red-stained reference plastics (50-1200 μm) photographed under three different microscope filters (UV: Filter System A S, BP 340-380 nm; blue: Filter System I3 S, BP 450-490 nm; and green: Filter system N2.1 S, BP 515-560 nm) (LEICA DM 1000). Image analysis to extract all RGB-values was performed using a macro in ImageJ. The supervised machine learning model (CART algorithm) trained by and validated with this dataset predicts with high accuracy the polymer types microplastics belong to, in a cost- and time-efficient way. RGB statistics of the most abundantly produced plastic polymers worldwide were compiled into the dataset. The statistics itself were calculated per reference particle as the 10th, 50th and 90th percentile as well as the mean of each of the three different color components extracted from all pixels laying along the maximum Feret diameter of that photographed particle. The dataset contains RGB-statistics calculated through image analysis of 210 plastic particles belonging to seven polymer types (Nylon, PE, PET, PP, PS, PUR and PVC) (30 particles/polymer type), where 168 particles (1/4) were randomly selected and used to serve as training data (24 particles/polymer) (worksheet tab ‘training data’), while the remaining 42 particles (1/5) were kept as independent validation data (6 particles/polymer type) (worksheet tab ‘training data’).

Scope
Themes:
Environmental quality/pollution
Keywords:
Marine, Fresh water, Brackish water, Fluorescence microscopy, Machine learning, Microplastics, Polymer identification method, RGB colour data, World

Geographical coverage

Parameter
RGB (Red, Green, Blue) colour component means and percentiles Methodology
RGB (Red, Green, Blue) colour component means and percentiles: Fluorescence microscopy combined with image analysis.

Contributors
Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center (ARC), moredata creator
Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie en Aquatische Ecologie (GhEnToxLab), moredata creator
Vlaams Instituut voor de Zee (VLIZ), moredata creator
Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw-, visserij en voedingsonderzoek (ILVO), moredata creator

Related datasets
Other relation:
RGB-statistics derived from Nile red-stained reference plastics for the construction of the PDM (Plastics Detection Model), more

Project
ANDROMEDA: Analysis techniques for quantifying nano-and microplastic particles and their degradation in the marine environment, more

Dataset status: Completed
Data type: Data
Data origin: Research: lab experiment
Metadatarecord created: 2021-08-23
Information last updated: 2021-09-28
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy