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Data of a long-term study on survival and development of successive generations of Mytilus galloprovincialis cryopreserved larvae
Citable as data publication
Heres, P.; Troncoso, J.; Paredes, E.; Grupo de Investigación en Ecología Costera (Uvigo-EcoCost): Spain; (2021); Data of a long-term study on survival and development of successive generations of Mytilus galloprovincialis cryopreserved larvae. Marine Data Archive. https://doi.org/10.14284/526

Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
The aim of this work was to study the improvement of cryopreservation protocol for Mytilus galloprovincialis larvae described in Paredes et al., 2021 studying different development stages, toxic and cryoprotection effects of Cryoprotecting Agent (CPAs), cooling and thawing rates and the use of sucrose (SUC) for CPA removal. Moreover, the capability of producing adult mussels from cryopreserved 72h-old D-larvae and potential long-term effects of cryopreservation through progenies was evaluated. more

Shellfish aquaculture needs the development of new tools for the improvement of good practices avoiding the reliance of natural spat collection leading to an increase of production more efficiently. Results showed that the selection of 72 h-old D-larvae for cryopreservation yielded around 75% of recovery, higher than 50% from trochophores. 10% Ethylene-Glycol + 0.4 M Trehalose in Filtered Sea Water (FSW) was the best CPA combination for D-larvae, as well as cooling at -1°C/min and water bath at 35 °C for thawing. SUC solutions did not improved larval recovery at any of the concentrations tested (p>0.05). At settlement, 5.26% of cryopreserved F1 larvae survived and over 70% were able to settle. F2 cryopreservation produced 0.15% of spat and settlement success varied from 35 to 50%. The delay of shell size showed on cryopreserved larvae was in declining throughout larval rearing without significant differences with controls from settlement point (p>0.05). Long-term experiments showed that it is possible to obtain adult mussels from cryopreserved larvae and this tool does not compromise the quality of following progenies, neither cryopreservation nor post-thawing development of them.

Scope
Themes:
Biology > Invertebrates
Keywords:
Marine, Cryopreservation, Cryoprotecting agents, D-larvae, Larval rearing, Mytilus galloprovincialis Lamarck, 1819

Temporal coverage
9 October 2018 - 5 April 2021

Taxonomic coverage
Mytilus galloprovincialis Lamarck, 1819 [WoRMS]

Parameter
Count of larvae

Contributors
University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera (EcoCost), moredata creator

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, more

Dataset status: Completed
Data type: Data
Data origin: Research: lab experiment
Metadatarecord created: 2021-10-18
Information last updated: 2021-10-18
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