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Chromosome and gene manipulation is the African Catfish, Clarias gariepinus.

More:  Institute 
Reference no: ID P103/3E890186, UNIT 103/22181
Period: October 1989 till September 1992
Status: Completed

Institute  Top 
  • Katholieke Universiteit Leuven; Departement Biologie; Afdeling Dierenecologie en -systematiek; Diversiteit en Systematiek van Dieren, more, co-ordinator

The aim is the molecular characterisation of fish genes. A constitutive promoter of a viral gene has been linked to the reporter genes lacZ or luciferase and was tested by means of transient and stable transgenesis. The max. of transient DNA replication and enzyme expression was observed between 8 and 24 h after injection of the embryo. The Mendelian inheritance of the introduced fusion gene was shown. In a series of parallel experiments the conditions for the cloning of African catfish have been established (gynogenesis).

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