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Novel microsatellite loci in the threatened European long-snouted seahorse (Hippocampus guttulatus) for genetic diversity and parentage analysis
Pardo, B.G.; López, A.; Martínez, P.; Bouza, C. (2007). Novel microsatellite loci in the threatened European long-snouted seahorse (Hippocampus guttulatus) for genetic diversity and parentage analysis. Conserv. Genet. 8(5): 1243-1245. dx.doi.org/10.1007/s10592-006-9241-7
In: Conservation Genetics. Springer: London; Dordrecht; Boston. ISSN 1566-0621, more
Peer reviewed article  

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Keyword
    Marine
Author keywords
    Seahorse Hippocampus guttulatus Microsatellites Enriched library Polymorphism Parentage

Authors  Top 
  • Pardo, B.G.
  • López, A.
  • Martínez, P.
  • Bouza, C.

Abstract
    The long-snouted seahorse Hippocampus guttulatus is one of the two European seahorse species. We describe the isolation of the first 12 microsatellite loci in this threatened species. These new markers were tested in non-invasive samples of 32 seahorses from NW Spain. The number of alleles ranged from 2 to 15 (mean: 6.3) and expected heterozygosity from 0.031 to 0.912 (mean: 0.500). All loci conformed to Hardy-Weinberg expectations and no genotypic disequilibrium was observed between any pair of loci. The theoretical exclusion probabilities for this set of loci, when no parental information exists or when one parent is known, were 0.973 and 0.998, respectively. This study indicates the usefulness of these novel loci for population analysis and kinship studies in Hippocampus guttulatus. Their potential application is extended to the other European seahorse species, since all loci were successfully cross-amplified in H. hippocampus.

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