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Mitochondria as a target for decavanadate toxicity in Sparus aurata heart
Soares, S.S.; Gutiérrez-Merino, C.; Aureliano, M. (2007). Mitochondria as a target for decavanadate toxicity in Sparus aurata heart. Aquat. Toxicol. 83(1): 1-9.
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X, more
Peer reviewed article  

Available in  Authors 

    Mitochondria; Oxygen consumption; Vanadium; Marine; Brackish water
Author keywords
    decameric vanadate; mitochondria; mitochondrial oxygen consumption;mitochondrial depolarization; vanadium

Authors  Top 
  • Soares, S.S.
  • Gutiérrez-Merino, C.
  • Aureliano, M.

    In a previous in vivo study we have reported that vanadium distribution, antioxidant enzymes activity and lipid peroxidation in Sparus aurata heart are strongly dependent on the oligomeric vanadate species being administered. Moreover, it was suggested that vanadium is accumulated in mitochondria, in particular when V10 was intravenously injected. In this work we have done a comparative study of the effects of V10 and monomeric vanadate (V1) on cardiac mitochondria from S. aurata. V10 inhibits mitochondrial oxygen consumption with an IC50 of 400 nM, while the IC50 for V1 is 23 μM. V10 also induced mitochondrial depolarization at very low concentrations, with an IC50 of 196 nM, and 55 μM of V1 was required to induce the same effect. Additionally, up to 5 μM V10 did inhibit neither F0F1-ATPase activity nor NADH levels and it did not affect respiratory complexes I and II, but it induced changes in the redox steady-state of complex III. It is concluded that V10 inhibits mitochondrial oxygen consumption and induces membrane depolarization more strongly than V1, pointing out that mitochondria is a toxicological target for V10 and the importance to take into account the contribution of V10 to the vanadate toxic effects.

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