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Biological and chemical approaches for the detection and identification of illegal estrogens in water based solutions
Noppe, H.; Arijs, K.; De Wasch, K.; Van Cruchten, S.; Poelmans, S.; Courtheyn, D.; Cobbaert, E.; Gillis, W.; Vanthemsche, P.; De Brabander, H.F.; Janssen, C.R.; Van Hoof, N. (2006). Biological and chemical approaches for the detection and identification of illegal estrogens in water based solutions, in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 59-72
In: Noppe, H. (2006). Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. PhD Thesis. Universiteit Gent. Faculteit Diergeneeskunde: Gent. ISBN 978-90-5864-100-7. 153 pp., more

Also published as
  • Noppe, H.; Arijs, K.; De Wasch, K.; Van Cruchten, S.; Poelmans, S.; Courtheyn, D.; Cobbaert, E.; Gillis, W.; Vanthemsche, P.; De Brabander, H.F.; Janssen, C.R.; Van Hoof, N. (2006). Biological and chemical approaches for the detection and identification of illegal estrogens in water based solutions. Vet. Res. Comm. 30(6): 577-585. dx.doi.org/10.1007/s11259-006-3326-0, more

Available in Authors 
    VLIZ: Open Repository 122325 [ OMA ]

Keywords
    Detection; Estrogens; Identification

Project Top | Authors 
  • Endocrine disruption in the Scheldt Estuary: distribution, exposure and effects, more

Authors  Top 
  • Noppe, H., more
  • Arijs, K., more
  • De Wasch, K., more
  • Van Cruchten, S.
  • Poelmans, S., more
  • Courtheyn, D.
  • Cobbaert, E.
  • Gillis, W.
  • Vanthemsche, P.
  • De Brabander, H.F., more
  • Janssen, C.R., more
  • Van Hoof, N.

Abstract
    Continuous production of new products used as growth promotors in animal husbandry, sports doping and products for body-building, requires residue laboratories to initiate research on developing a strategy for the identification of ‘unknown’ components. In this study, a strategy for elucidating the identity, the structure and the possible effects of illegal estrogenic compounds in an unidentified water based solution is presented. To obtain complete information on the composition and activity of the unidentified product a multi-disciplinary approach was needed. A case-study is described with a ‘solution X’ found during a raid. At first, in vivo techniques (animal trials with mice, anatomical and histological research) were combined with in vitro techniques (the yeast estrogenic screen (YES)). In a later state of the investigation, HPLC fractionation, liquid chromatography-multiple mass spectrometry (LCMS- MS) and gas chromatography-multiple mass spectrometry (GC-MS-MS) were used. Finally, the identity of ‘solution X’ was confirmed in a low concentration range (10 ng.l-1 E1 and 400 ng.l-1 EE2).

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