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Multi-analyte approach for the determination of ng.l-1 amounts of steroid hormones in unidentified aqueous samples
Noppe, H.; Verheyden, K.; Gillis, W.; Courtheyn, D.; Vanthemsche, P.; De Brabander, H.F. (2007). Multi-analyte approach for the determination of ng.l-1 amounts of steroid hormones in unidentified aqueous samples. Anal. Chim. Acta 586(1-2): 22-29. dx.doi.org/10.1016/j.aca.2006.08.048
In: Analytica Chimica Acta. Elsevier: New York; Amsterdam. ISSN 0003-2670, more
Peer reviewed article  

Also published as
  • Noppe, H.; Verheyden, K.; Gillis, W.; Courtheyn, D.; Vanthemsche, P.; De Brabander, H.F. (2006). Multi-analyte approach for the determination of ng.l-1 amounts of steroid hormones in unidentified aqueous samples, in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 71-86, more

Available in Authors | Dataset 
    VLIZ: Open Repository 280313 [ OMA ]

Keywords
    Analytical techniques; Identification; Steroids; Marine; Brackish water; Fresh water

Authors  Top | Dataset 
  • Noppe, H., more
  • Verheyden, K., more
  • Gillis, W.
  • Courtheyn, D.
  • Vanthemsche, P.
  • De Brabander, H.F., more

Abstract
    Since the 70s, many analytical methods for the detection of illegal growth promotors, such as thyreostats, anabolics, ß-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng.l-1 levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the ‘black’ market. For this, extraction was performed with Bakerbond C18 speedisk, a technique commonly used in environmental analysis. After fractionation, 4 fractions were collected using a methanol:water gradient program. Gas Chromatography coupled to Electron Impact multiple Mass Spectrometry (GC-EI-MS-MS) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, Gas Chromatography coupled to Negative Chemical Ionization Mass Spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by Liquid Chromatography coupled to Electrospray Ionization multiple Mass Spectrometry (LC-ESI-MS-MS). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng.l-1 level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples.

Dataset
  • Chemical analysis of estrogens, organochlorine and organonitrogen pesticides as possible endocrine disruptors Scheldt-estuary, more

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