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Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea
Rhee, J.-S.; Lee, Y.-M.; Hwang, D.-S.; Won, E.-J.; Raisuddin, S.; Shin, K.-H.; Lee, J.-S. (2007). Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. Aquat. Toxicol. 83(2): 104-115. dx.doi.org/10.1016/j.aquatox.2007.03.015
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X, more
Peer reviewed article  

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Keywords
    Biological stress; Biomarkers; Glutathione; Heavy metals; Metallothioneins; Nereis (Neanthes) succinea (Leuckart, 1847) [WoRMS]; Polychaeta [WoRMS]; Marine

Authors  Top 
  • Rhee, J.-S.
  • Lee, Y.-M.
  • Hwang, D.-S.
  • Won, E.-J.
  • Raisuddin, S.
  • Shin, K.-H.
  • Lee, J.-S.

Abstract
    We cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed Escherichia coli and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on N. succinea GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that N. succinea GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of N. succinea GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination.

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