|Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus)|Chen, S.-L.; Sha, Z.X.; Ye, H.-Q.; Liu, Y.; Tian, Y.-S.; Hong, Y.; Tang, Q.-S. (2007). Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus). Mar. Biotechnol. 9(1): 82-91. dx.doi.org/10.1007/s10126-006-6050-1
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, more
Embryonic stem cells; Lateolabrax japonicus (Cuvier, 1828) [WoRMS]; Marine
|Authors|| || Top |
- Chen, S.-L.
- Sha, Z.X.
- Ye, H.-Q.
- Liu, Y., more
- Tian, Y.-S.
- Hong, Y.
- Tang, Q.-S.
A stable GFP-expressing (GFP+LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP+LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP+LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP+LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP+LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP+LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.