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Expression of masu salmon Δ5-desaturase-like gene elevated EPA and DHA biosynthesis in zebrafish
Yoshizaki, A.G.; Kiron, V.; Satoh, S.; Takeuchi, T. (2007). Expression of masu salmon Δ5-desaturase-like gene elevated EPA and DHA biosynthesis in zebrafish. Mar. Biotechnol. 9(1): 92-100.
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, more
Peer reviewed article  

Available in Authors 

    Transgenic; Marine

Authors  Top 
  • Yoshizaki, A.G.
  • Kiron, V.
  • Satoh, S.
  • Takeuchi, T.

    Farmed fish could substitute for marine capture fish as a source of fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) beneficial for human health; however, they require these compounds in their diets. In the present study on a model fish species, we modified the EPA/DHA biosynthesis pathway by overexpression of masu salmon Δ5-desaturase-like gene in zebrafish to increase its ability to synthesize EPA and DHA. Expression of this gene in transgenic fish fed a commercial diet and Artemia helped to improve their EPA content by 1.21-fold and DHA by 1.24-fold. In similar fish that were fed only Artemia the increments were 1.14-fold for EPA and 1.13-fold for DHA, compared with nontransgenic fish. In contrast, eicosatetraenoic acid content decreased, as it is a substrate of Δ5-desaturase, while the total lipid remained constant. The results demonstrated that masu salmon Δ5-desaturase is functional in zebrafish and can modify its fatty acid metabolic pathway. The technique could be applied to farmed fish to generate a nutritionally richer product for human consumption.

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