IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Methods used to reveal genetic diversity in the colony-forming prymnesiophytes Phaeocystis antarctica, P. globosa and P. pouchetii : preliminary results
Gaebler, S.; Hayes, P.K.; Medlin, L.K. (2007). Methods used to reveal genetic diversity in the colony-forming prymnesiophytes Phaeocystis antarctica, P. globosa and P. pouchetii : preliminary results. Biogeochemistry 83(1-3): 19-27. dx.doi.org/10.1007/s10533-007-9084-4
In: Biogeochemistry. Springer: Dordrecht; Lancaster; Boston. ISSN 0168-2563, more
Peer reviewed article  

Also published as
  • Gaebler, S.; Hayes, P.K.; Medlin, L.K. (2007). Methods used to reveal genetic diversity in the colony-forming prymnesiophytes Phaeocystis antarctica, P. globosa and P. pouchetii : preliminary results, in: Van Leeuwe, M.A. et al. (Ed.) Phaeocystis, major link in the biogeochemical cycling of climate-relevant elements. Biogeochemistry, 83(1-3): pp. 19-27, more

Available in Authors 
Document type: Conference paper

Keywords
    Aflp; Genetic diversity; Phaeocystis Lagerheim, 1893 [WoRMS]; Phaeocystis antarctica Karsten, 1905 [WoRMS]; Phaeocystis globosa Scherffel, 1899 [WoRMS]; Phaeocystis pouchetii (Hariot) Lagerheim, 1896 [WoRMS]; Marine

Authors  Top 
  • Gaebler, S.
  • Hayes, P.K.
  • Medlin, L.K., more

Abstract
    Previous work on the genetic diversity of Phaeocystis used ribosomal DNA and internal transcribed spacer (ITS) sequence analyses to show that there is substantial inter- and intraspecific variation within the genus. First attempts to trace the biogeographical history of strains in Antarctic coastal waters were based on a comparison of ITS sequences. To gain deeper insights into the population structure and bloom dynamics of this microalga it is necessary to quantify the genetic diversity within populations of P. antarctica from different locations (i.e., each of the three major gyres in the Antarctic continental waters) and to calculate the gene flow between them. Here we describe methods to quantify genetic diversity and our preliminary results for P. antarctica in comparison to two other colonial species: P. globosa and P. pouchetii. For this study of genetic diversity, two fingerprinting techniques were used. First, amplified fragment-length polymorphisms (AFLPs) were established as a pre-screening tool to assess clone diversity and to select divergent clones prior to physiological investigations. Second, the more-powerful microsatellite markers were established to assess population structure and biogeography more accurately. Results show differences in the AFLP patterns between isolates of P. antarctica from different regions, and that a wide variety of microsatellite motifs could be obtained from the three Phaeocystis species.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors