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A simple chromosome spread technique for unarmored dinoflagellates and implications of polyploidy in algal cultures
Loper, C.L.; Steidinger, K.A.; Walker, L.M. (1980). A simple chromosome spread technique for unarmored dinoflagellates and implications of polyploidy in algal cultures. Trans. Am. Microsc. Soc. 99(3): 343-346
In: Transactions of the American Microscopical Society. Blackwell Publishing: Lawrence, Kan., etc.,. ISSN 0003-0023, more
Peer reviewed article  

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Keywords
    Analysis; Chromosomes; Ptychodiscus brevis (C.C.Davis) Steidinger, 1979 [WoRMS]

Authors  Top 
  • Loper, C.L.
  • Steidinger, K.A.
  • Walker, L.M.

Abstract
    Chromosome counts can be used to verify stages of dinoflagellate life cycles. A simple method involving cell lysis, heat, desiccation, and staining has been effective in studying Ptychodiscus brevis chromosomes. Two vegetative count ranges were determined with this technique: (1) a haploid number of 121 3 for field and recent culture specimens; and (2) a diploid number of 240 6 for a 25-yr old culture. The latter probably is a result of autodiploidy during extended culture conditions. Polyploidy may be occurring in many long-established algal cultures.

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