Discrimination of the northeast Atlantic lesser sandeels, Ammodytes marinus, Ammodytes tobianus and Gymnammodytes semisquamatus by protein electrophoresis
Donaghy, M.J.; Verspoor, E.; Youngson, A.F. (1995). Discrimination of the northeast Atlantic lesser sandeels, Ammodytes marinus, Ammodytes tobianus and Gymnammodytes semisquamatus by protein electrophoresis. Fish. Res. 23(1-2): 1-9. https://dx.doi.org/10.1016/0165-7836(94)00340-3
In: Fisheries Research. Elsevier: Amsterdam. ISSN 0165-7836; e-ISSN 1872-6763, more
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| Keywords |
Analytical techniques > Electrophoresis
Chemical compounds > Organic compounds > Proteins
Ammodytes marinus Raitt, 1934 [WoRMS]; Ammodytes tobianus Linnaeus, 1758 [WoRMS]; Gymnammodytes semisquamatus (Jourdain, 1879) [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Donaghy, M.J.
- Verspoor, E.
- Youngson, A.F.
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| Abstract |
The northeast Atlantic lesser sandeels Ammodytes marinus Raitt, Ammodytes tobianus L. and Gymnammodytes semisquamatus Jourdain were screened by starch gel electrophoresis for species-specific enzyme variation. Morphological separation of the genera is reliable but distinguishing the two Ammodytes species is problematic, particularly in smaller specimens. The analysis revealed a number of diagnostic isozymes. Two, MEP-1 and MEP-2, appear particularly suitable for discriminating the three species. These isozymes show no intraspecific variation, can be typed using muscle, are relatively stable in frozen tissue, and can be resolved using a single gel and histochemical stain. The use of these diagnostic isozymes will facilitate species discrimination in fisheries assessment work and in studies of the ecological differences among the species. |
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