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Effects of preservation on dry- and ash-free dry weight biomass of some common aquatic macro-invertebrates
Leuven, R.S.E.W.; Brock, T.C.M.; van Druten, H.A.M. (2004). Effects of preservation on dry- and ash-free dry weight biomass of some common aquatic macro-invertebrates. Hydrobiologia 127(2): 151-159
In: Hydrobiologia. Springer: The Hague. ISSN 0018-8158, more
Peer reviewed article  

Available in Authors 

Keywords
    Preservation (organisms); Fresh water

Authors  Top 
  • Leuven, R.S.E.W.
  • Brock, T.C.M.
  • van Druten, H.A.M.

Abstract
    The effect of preservation methods on dry weight (DW) and ash-free dry weight (AFDW) of Radix peregra (Gastropoda), Asellus aquaticus (Isopoda), Erpobdella octoculata (Hirudinea) and Glyptotendipes sp. (Chironomidae) was studied. Ethanol, formaldehyde, and Bouin were used as preservative. In case of preservation of macro-invertebrates in ethanol substancial changes in DW and AFDW biomass were observed. In the four different taxa the loss in DW varied between 7.2–21.9% after a 3 month preservation period in 70% ethanol. A comparatively small range in AFDW loss (16.2–19.7%) was found. Changes in DW and AFDW biomass during preservation were significantly affected by the duration of the preservation, by temperature, light conditions and the volume of the preservative. The changes in AFDW were also significantly affected by the concentration of the preservatives. Preservation in 10% formaldehyde did not cause significant changes in DW and AFDW biomass.

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