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Sperm cryoconservation in Anarhichas sp., endangered cold-water aquaculture species with internal fertilization
Le François, N.R.; Lamarre, S.G.; Tveiten, H.; Blier, P.U.; Bailey, J. (2008). Sperm cryoconservation in Anarhichas sp., endangered cold-water aquaculture species with internal fertilization. Aquacult. Int. 16(3): 273-279. https://dx.doi.org/10.1007/s10499-007-9137-7
In: Aquaculture International. Springer: London. ISSN 0967-6120; e-ISSN 1573-143X, more
Peer reviewed article  

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Keywords
    Agents > Antifreezes
    Aquaculture
    Cells > Sexual cells > Sperm
    Chemical compounds > Organic compounds > Proteins
    Cryopreservation
    Endangered species
    Internal fertilization
    Taxa > Species > Commercial species
    Anarhichadidae Bonaparte, 1835 [WoRMS]; Anarhichas minor Olafsen, 1772 [WoRMS]
    Marine/Coastal
Author keywords
    Anarhichas; cryopreservation; internal fertilization; wolffish;antifreeze proteins

Authors  Top 
  • Le François, N.R.
  • Lamarre, S.G.
  • Tveiten, H.
  • Blier, P.U.
  • Bailey, J.

Abstract
    This study contributes to the identification of an adequate cryopreservation medium for wolffishes sperm (Anarhichas minor and A. lupus). Our results also confirm the presence of antifreeze proteins in the seminal fluid of the Atlantic wolffish (Anarhichas lupus) that could facilitate the process of cryoconservation in association with increased post-thaw motility and fertilization rates. Cryo-Fish, a commercial diluent was the most efficient in our trials, with 80% motility post-thaw.

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