|Norchlorotestosterone acetate: An alternative metabolism study and GC-MS2 analysis in kidney fat, urine, and faeces|
Van Hoof, N.; De Wash, K.; Poelmans, S.; Bruneel, D.; Spruyt, S.; Noppe, H.; Janssen, C.R.; Courtheyn, D.; De Brabander, H.F. (2004). Norchlorotestosterone acetate: An alternative metabolism study and GC-MS2 analysis in kidney fat, urine, and faeces. Chromatographia (Wiesb.) 59: S85-S93
In: Chromatographia. Vieweg Verlag: Heidelberg; Berlin. ISSN 0009-5893, more
Atmospheric pressure; Chemical analysis; Chlorine organic compounds; Electron impact; Feces; Gas chromatography; HPLC; Kidneys; Mass spectrometry; Mass spectrometry; Mass spectrometry; Metabolism; Metabolites; Qualitative analysis; Steroids; Urine; Arthropoda [WoRMS]; Crustacea [WoRMS]; Invertebrata; Mysidacea [WoRMS]; Neomysis integer (Leach, 1814) [WoRMS]; Fresh water
|Authors|| || Top |
- Van Hoof, N.
- De Wash, K.
- Poelmans, S., more
- Bruneel, D.
- Spruyt, S.
- Noppe, H., more
- Janssen, C.R., more
- Courtheyn, D.
- De Brabander, H.F., more
Norchlorotestosterone acetate (NCITA) is an anabolic steroid which resembles chlorotestosterone acetate. It cannot yet be detected by routine methods used for anabolic steroids, because there is no knowledge of its metabolic pathway. The invertebrate Neomysis integer has been used as an alternative model to study the metabolism of NCITA. The experimental results indicated the presence of 4-norchloroandrost-4-ene-17-ol-3-one (NCIT) and 4-norchloroand-rost-4-ene-3,17-dione (NorCIAD) as possible metabolites of NCITA. Subsequently NCITA and the synthesised metabolites NCIT and NorCIAD were incorporated into the routine multi-residue method for detection of anabolic steroids in kidney fat, urine, and faeces.