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Induction of vitellogenesis in 17 alpha-ethinylestradiol-exposed rainbow trout (Oncorhynchus mykiss): a method comparison
Verslycke, T.; Vandenbergh, G.F.; Versonnen, B.; Arijs, K.; Janssen, C.R. (2002). Induction of vitellogenesis in 17 alpha-ethinylestradiol-exposed rainbow trout (Oncorhynchus mykiss): a method comparison. Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol. 132(4): 483-492
In: Comparative Biochemistry and Physiology. C. Comparative Pharmacology and Toxicology. Pergamon: Oxford; New York. ISSN 0742-8413, more
Peer reviewed article  

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Keywords
    Calcium; Ethinylestradiol; Oncorhynchus mykiss (Walbaum, 1792) [WoRMS]; Fresh water

Authors  Top 
  • Verslycke, T., more
  • Vandenbergh, G.F.
  • Versonnen, B., more

Abstract
    Juvenile rainbow trout, Oncorhynchus mykiss, were exposed to the synthetic estrogen 17alpha-ethinylestradiol (EE2) through injection (1, 10, 25 and 50 mug EE2/g fish/week) and via water exposure (1, 10 and 100 ng EE2/1). After seven (injection and water exposure) and 14 days (only for water exposure), blood and plasma vitellogenin concentrations were quantified using indirect endpoints, i.e. plasma alkaline-labile phosphorus (ALP), plasma protein and plasma calcium. In addition, the relative gonad (GSI) and liver weight (HSI) were recorded. Actual plasma vitellogenin concentrations were measured with an enzyme immunoassay. Only fish injected with 50 mug EF2/g fish had a significantly higher gonad weight. No concentration-dependent changes in the HSI were detected in fish exposed via the water, but a significant dose-dependent increase of the HSI was observed in fish injected with EE2. Exposure of rainbow trout to EE2 had a significant effect on all tested plasma parameters. Plasma protein, phosphoprotein and calcium concentrations were significantly higher after two weeks exposure to 100 ng EE2/1. Fish injected with 10, 25 and 50 mug EE2/g fish exhibited increased plasma protein concentrations after 1 week. Compared to the controls, plasma ALP and calcium levels were significantly higher in all injected fish. A significant and positive correlation was observed between all three plasma parameters and between these indirect parameters and the actual plasma vitellogenin concentrations. These findings indicate that both the plasma ALP and the plasma calcium assay have a similar sensitivity as that of available antibody-based assays (EIA), at least in EE2 exposure studies, and thus these assays can provide a rapid, simple and cost-effective alternative to available immunoassays

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