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Alcian blue: a quantitative aqueous assay for algal acid and sulfated polysaccharides
Ramus, J. (1977). Alcian blue: a quantitative aqueous assay for algal acid and sulfated polysaccharides. J. Phycol. 13: 345-348
In: Journal of Phycology. Blackwell Science: New York. ISSN 0022-3646, more
Peer reviewed article  

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    Acids; Agar; Alginic acid; Assaying; Carrageenan; Pectin; Polysaccharides; Marine

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  • Ramus, J.

    Alcian Blue, a cationic copper phthalocyanine dye, complexes with the anionic carboxyl and half-ester sulfate groups of acidic algal polysaccharide in aqueous solution to form an insoluble precipitate. The quantity of dye removed from solution is proportional to the quantity of polyanion in solution, and this principle forms the basis for the quantitative determination of acid and/or sulfated algal polysaccharides. The assay is linear between 0 and 100 µg/ml agar, alginic acid, carrageenan, pectin and Porphyridium aerugineum Geit. polysaccharide. In addition, the technique is used to determine the anion density of acid polysaccharides on a molar or weight equivalency basis.

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