IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Print this page

Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5
Kime, D.E.; Lee, E.Y.; Kim, H.S. (2009). Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar. Biotechnol. 11(1): 10-16. https://dx.doi.org/10.1007/s10126-008-9114-9
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, more
Peer reviewed article  

Available in  Authors 

Keywords
    Oligosaccharides
    Products > Fishery products > Processed fishery products > Seaweed products > Alginates
    Marine/Coastal
Author keywords
    Alginate lyase; Alginate oligosaccharide; Streptomyces sp ALG-5;Unsaturated uronate

Authors  Top 
  • Kime, D.E.
  • Lee, E.Y.
  • Kim, H.S.

Abstract
    A marine bacterium was isolated from seaweeds for its ability to degrade alginate. Analysis of 16S ribosomal DNA sequence and chemotaxonomic characterizations revealed that the strain belongs to Streptomyces. The alginate lyase gene of Streptomyces sp. ALG-5 was cloned by using PCR with the specific primer designed from homologous nucleotide sequences. The consensus sequences of N-terminal YXRSELREM and C-terminal YFKAGXYXQ were conserved in the ALG-5 alginate lyase gene. The recombinant alginate lyase was purified by using Ni-Sepharose affinity chromatography. The alginate lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The degraded products were determined to be di-, tri-, tetra- and pentasaccharides by using BioGel P-2 gel filtration chromatography and ionization mass spectroscopy method.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors