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Identification of sulphate-reducing ectosymbiotic bacteria from anaerobic ciliates using 16S rRNA binding oligonucleotide probes
Fenchel, T.; Ramsing, N.B. (1992). Identification of sulphate-reducing ectosymbiotic bacteria from anaerobic ciliates using 16S rRNA binding oligonucleotide probes. Arch. Microbiol. 158(6): 394-397
In: Archives of Microbiology. Springer: Heidelberg; Berlin. ISSN 0302-8933, more
Peer reviewed article  

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Keywords
    Anaerobes; Ciliates; Symbionts; Caenomorpha levanderi Kahl, 1927 [WoRMS]; Metopus contortus (Quennerstedt, 1867) [WoRMS]; Marine

Authors  Top 
  • Fenchel, T., more
  • Ramsing, N.B.

Abstract
    The identity of ectosymbiotic bacteria of some marine, free-living anacrobic ciliates (Metopus contortus, Caenomorpha levanderi and Parablepharisma sp.) was studied using fluorescent-dye-conjugated oligonucleotides complementary to short sequence elements of 16S ribosomal RNA. The ectosymbiotic bacteria of all species hybridized with a eubacterial probe and those of the two former mentioned species hybridized with a general probe for sulphate-reducing bacteria, but not to a probe specific for Desulfobacter. The results support indirect evidence suggesting that ectosymbiotic bacteria of anaerobic ciliates are sulphate-reducers which depend on host metabolites for substrates.

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