Preparation of single-celled marine dinoflagellates for electron microscopy
Truby, E.W. (1997). Preparation of single-celled marine dinoflagellates for electron microscopy. Microsc. Res. Tech. 36(4): 337-340
In: Microscopy Research and Technique. Wiley-Liss: New York, NY. ISSN 1059-910X; e-ISSN 1097-0029, more
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| Abstract |
Electron microscopy has been used successfully to study and identify single-celled marine dinoflagellates including parasitic ones and others, such as those that cause red tide. Delicate cells can be preserved for scanning electron microscopy with a combined glutaraldehydeosmium tetroxide mixture that is adjusted for the osmolality of the medium. The protocol allows resolution of fine morphological features. Preservation for transmission electron microscopy can be accomplished with a standard glutaraldehyde fixation and osmium-tetroxide post-fixation in a suitable buffer, but again, the osmolality of the mixture must be adjusted. The protocol allows ultrastructural resolution of vesiculated cells and has been modified for small sample sizes |
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