|Comparison of gene expression profiles of Fenneropenaeus chinensis challenged with WSSV and Vibrio|
Wang, B.; Li, F.; Luan, W.; Xie, Y.; Zhang, C.; Luo, Z.; Gui, L.; Yan, H.; Xiang, J. (2008). Comparison of gene expression profiles of Fenneropenaeus chinensis challenged with WSSV and Vibrio. Mar. Biotechnol. 10(6): 664-675
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, more
Cdna array; Fenneropenaeus chinensis (Osbeck, 1765) [WoRMS]; Vibrio O.F.Müller, 1773 [WoRMS]; Marine
|Authors|| || Top |
- Wang, B.
- Li, F.
- Luan, W.
- Xie, Y.
- Zhang, C.
- Luo, Z.
- Gui, L.
- Yan, H.
- Xiang, J.
Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.