|Axenic culture and uptake of dissolved organic substances by the marine nematode, Rhabditis marina Bastian|
Tietjen, J.H.; Lee, J.J. (1975). Axenic culture and uptake of dissolved organic substances by the marine nematode, Rhabditis marina Bastian. Cah. Biol. Mar. 16(5): 685-694
In: Cahiers de Biologie Marine. Station Biologique de Roscoff: Paris. ISSN 0007-9723, more
Cultures; Nutrients (mineral); Nutritional requirements; Rhabditis (Pellioditis) marina (Bastian, 1865) Andrassy, 1984 [WoRMS]; Marine
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Monoxenic R. marina were freed from the associated bacteria (Pseudomonas sp) by aseptic washing and transfer through anti-biotic-containing media. R. marina was maintained axenically on Grace's Insect media and a marine salt base. The addition of casamino acids, hemoglobin and crude natural materials rich in lipids were fond to stimulate growth and reproduction. Lipid rich materials such as TEM (trienthyleneamine) and TWEEN 80 were consistently stimulatory, and replaced the amino acid mixture when supplemented with whole egg powder. Mixtures of various fatty acids were able to replace the undefined lipid mixtures. It is suspected that R. marina has fewer fatty acid synthetic abilities than other free-living nematodes which have been studied. The uptake of several dissolved organic substances by R. marina was examined. No uptake of 14C-labelled organic substances from solution was observed, even at high (20 mM) concs. The addition of small (0.5-10µ ) latex microspheres to the experimental medium however, resulted in uptake of label. This suggests that the major nutritional pathways for R. marina is probably through particulate material, such as bacteria and perhaps algae.