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Effects of oil on digestive cells in mussels: Quantitative alterations in cellular and lysosomal structure
Lowe, D.M.; Moore, M.N.; Clarke, K.R. (1981). Effects of oil on digestive cells in mussels: Quantitative alterations in cellular and lysosomal structure. Aquat. Toxicol. 1(3-4): 213-226
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X, more
Peer reviewed article  

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Keywords
    Image analysis; Lysosomes; Oils; Mytilus edulis Linnaeus, 1758 [WoRMS]; Marine

Authors  Top 
  • Lowe, D.M.
  • Moore, M.N.
  • Clarke, K.R.

Abstract
    Structural changes were observed in the digestive tubule epithelial cells of Mytilus edulis following long-term exposure to the water accommodated fraction (WAF) of North Sea crude oil (30 μg · l−1 total oil derived aromatic hydrocarbons). The changes observed involved a reduction in the height of the digestive cells beyond that demonstrated in a normal feeding cycle. In addition there was a loss of the normal synchrony of the digestive cells to a point where nearly all the tubules exhibited an appearance similar to that which is usually termed ‘reconstituting’. These alterations were quantified using an image analysis technique and the mean height of the digestive cells used as an index of digestive function or state. Long-term exposure also induced a radical alteration of the structure of secondary lysosomes within the digestive cells, resulting in the formation of large lysosomes, believed to be autolysosomes. Stereological analyses showed that these lysosomes are reduced in numbers and greatly increased in volume in comparison with controls. There is a concomitant increase in surface area of lysosomes per unit volume of digestive cell compared with control conditions. These alterations are indicative of fundamental changes in secondary lysosomal function involving an autophagic response to oil derived hydrocarbons. which would contribute to the reduction of digestive cell cytoplasm.These cellular alterations are discussed in terms of their use as indices of cell injury, in response to oil.

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