|Novel cytotoxic peptides from the tropical marine cyanobacterium Hormothamnion enteromorphoides. 1. Discovery, isolation and initial chemical and biological characterization of the hormothamnins from wild and cultured material|
Gerwick, W.H.; Mrozek, C.; Moghaddam, M.F.; Agarwal, S.K. (1989). Novel cytotoxic peptides from the tropical marine cyanobacterium Hormothamnion enteromorphoides. 1. Discovery, isolation and initial chemical and biological characterization of the hormothamnins from wild and cultured material. Experientia 45(2): 115-121
In: Experientia. Birkhäuser: Basel; Boston; Berlin. ISSN 0014-4754, more
Blue green algae; Cyanobacteria; Cytotoxicity; Natural products; Marine
|Authors|| || Top |
- Gerwick, W.H.
- Mrozek, C.
- Moghaddam, M.F.
- Agarwal, S.K.
A Caribbean cyanobacterium, Hormothamnion enteromorphoides, was found to produce a complex mixture of ichthyotoxic peptides, perhaps explaining the apparent absence of predation upon these potentially palatable life forms. Bioassay-guided fractionation was used to isolate these toxic and antimicrobial natural products, and a variety of techniques including HR FAB mass spectrometry, 2D-NMR, traditional hydrolysis-amino acid analysis, and several chemical reactions were used to define the basic structural features of the major peptide, hormothamnin A. Hormothamnin A is a cyclic undecapeptide containing six common and five uncommon or new amino acid residues. HPLC analyses indicate that the relative proportions of these peptide natural products remain relatively constant between different collection locations and years, however, they do vary seasonally. Clonal isolates of this cyanobacterium in culture produce the full spectrum of toxic peptides.