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Vital staining of bivalve mollusk shells with alizarin sodium monosulfonate
Hidu, H.; Hanks, J.E. (1968). Vital staining of bivalve mollusk shells with alizarin sodium monosulfonate. Proceedings of the National Shellfisheries Association 58: 37-41
In: Proceedings of the National Shellfisheries Association. Economy Printing: Easton. ISSN 0077-5711, more

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Keywords
    Biomarkers; Mercenaria mercenaria; Staining; Mya arenaria Linnaeus, 1758 [WoRMS]; Marine

Authors  Top 
  • Hidu, H.
  • Hanks, J.E.

Abstract
    Alizarin sodium monosulfonate was used for vital staining of shells of larval and post metamorphic hard and soft shelled clams, Mercenana mercenaria and Mya arenaria In post metamorphic clams a week-long immersion in 5 to 20 ppm alizarin resulted in peripheral and medial deposition of red shell material Larval M. mercenaria, when subjected to 0.25 to 0.75 ppm alizarin for 48 hours, produced distinctive check marks on shells which may have value in marking. The long persistence (at least 18 months in the present study) of metabolically induced red shell coloration gives this method an advantage over more superficial marking techniques used in ecological research.

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