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Marine vibrios isolated from water along the Dutch coast
Golten, C.; Scheffers, W.A. (1975). Marine vibrios isolated from water along the Dutch coast. Neth. J. Sea Res. 9(3-4): 351-364
In: Netherlands Journal of Sea Research. Netherlands Institute for Sea Research (NIOZ): Groningen; Den Burg. ISSN 0077-7579, more
Peer reviewed article  

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  • Golten, C.
  • Scheffers, W.A.

    Water samples, collected along the Dutch coast, were examined for the presence of marine vibrios. The procedure included enrichment in trypticase soy broth with 7% (w/v) NaCl at 41°C ± 1°C for 18 to 48 h, followed by isolation on modified thiosulphate citrate bile salts sucrose agar (TCBS agar), containing 3% (w/v) NaCl and incubated at 41°C ± 1°C for 18 to 24h. The procedure procured moderate recovery from 1 to 20 viable cells, good recovery from more than 20 viable cells of Vibrio parahaemolyticus in 50 ml-samples of sea water, and good recovery from 3 to 20 viable cells of V. alginolyticus in 50 ml-samples. From 67 samples (50 ml) of sea water, estuarine water, and other saline or brackish inshore water, collected along the coast in a period of maximum water temperatures, 10 samples yielded no vibrios. From all other samples (85%), Vibrio parahaemolyticus biotype 2 ( alginolyticus) was isolated; 4 of these isolates were methyl red-positive, and 2 other isolates were arabinose-positive. Vibrio parahaemolyticus biotype 1 (parahaemolyticus ) was not detected in any of the samples.

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