|Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting|
|Zhang, Y.; Dong, J.; Yang, B.; Ling, J.; Wang, Y.; Zhang, S. (2009). Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting. Sci. Mar. (Barc.) 73(3): 487-498|
|In: Scientia Marina (Barcelona). Consejo Superior de Investigaciones Científicas. Institut de Ciènces del Mar: Barcelona. ISSN 0214-8358, more|
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Bacterial community structure and the relationship between environmental variables and microbial communities
in the surface sediments of tropical mangrove ecosystems were investigated in Sanya, Hainan Island, China. Profiles of bacterial communities were generated using culture-independent PCR-denaturing gradient gel electrophoresis (DGE), and the results were interpreted with multivariate statistical analysis. Findings suggested that microbial communities varied with sample collection sites and seasons. The samples collected from the same sample sites at the same time had more similar microbial communities except samples SH3 and AB5, which also had unique sediment quality. Canonical correspondence analysis (CCA) revealed that the organic carbon concentration of the sediments accounted for a significant amount of the variability in the bacterial community composition. Phylogenetic analysis was used to identify the major groups of the predominant bacterial phylotypes. 16S rRNA gene-V3 fragments from 17 individual DGE bands were sequenced and the
corresponding bacteria were found in mangrove sediments for the first time based on BLAST results. Predominant bacterial phylotypes clustered with various taxonomic groups, including Proteobacteria, Bacteroidetes, Gemmatimonadetes, Actinobacteria and Firmicutes.