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The route of entry and progression of infectious haematopoietic necrosis virus in Oncorhynchus mykiss (Walbaum): a sequential immunohistochemical study
Drolet, B.S.; Rohovec, J.S.; Leong, J.C. (1994). The route of entry and progression of infectious haematopoietic necrosis virus in Oncorhynchus mykiss (Walbaum): a sequential immunohistochemical study. J. Fish Dis. 17(4): 337-347
In: Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne. ISSN 0140-7775, more
Peer reviewed article  

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Keywords
    Alkaline phosphatase; Circulatory system; Fish diseases; Gastrointestinal tract; Gills; Histochemistry; Immunohistochemistry; Organs; Pathology; Tissue culture; Viral diseases; Oncorhynchus mykiss (Walbaum, 1792) [WoRMS]; Marine; Brackish water; Fresh water

Authors  Top 
  • Drolet, B.S.
  • Rohovec, J.S.
  • Leong, J.C.

Abstract
    Steelhead trout, Oncorhynchus mykiss (Walbaum), fry were experimentally infected with infectious haematopoietic necrosis virus (IHNV) Round Butte 1983 (Type 1). Fry were sampled daily, before and during the epizootic. Fish tissues were tested for infectious virus by tissue culture assay and for IHNV nucleocapsid protein by alkaline phosphatase immunohistochemistry (APIH). The progression of virus through the tissues was followed by APIH until the fourteenth day. Viral infection progressed from two major sites: from the gills into the circulatory system; and from the oral region into the gastrointestinal tract and then into the circulatory system. Once in the blood, virus was disseminated to virtually every organ. Progression of IHNV within and between organs is discussed.

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