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Artificial oocyte maturation in Patella depressa and Patella vulgata using NaOH-alkalinized seawater
De souza, R.A.; Tyler, P.A.; Hawkins, S.J. (2009). Artificial oocyte maturation in Patella depressa and Patella vulgata using NaOH-alkalinized seawater. Mar. Biol. Res. 5(5): 503-510. https://hdl.handle.net/10.1080/17451000802603645
In: Marine Biology Research. Taylor & Francis: Oslo; Basingstoke. ISSN 1745-1000, more
Peer reviewed article  

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Keyword
    Marine
Author keywords
    Fertilization; larval rearing; mollusc; oocyte maturation; Patella; pH

Authors  Top 
  • De souza, R.A.
  • Tyler, P.A., more
  • Hawkins, S.J.

Abstract
    Laboratory investigations of environmental factors controlling early development and larval survival of free-spawning marine invertebrates are often limited by the difficulty of inducing spawning under laboratory conditions. Patellid gastropod species must be dissected in order to obtain the gametes from the gonads, often yielding unfertilizable immature eggs. The artificial maturation of oocytes through NaOH-alkalinization methods was investigated in order to improve laboratory experiments on larval ecology of Patella depressa and Patella vulgata. Maturation was accelerated above pH 9.0. Alkalinizing oocytes at pH 9.5 significantly enhanced in vitro fertilization rates, especially at higher sperm concentrations. pH 10 was harmful to eggs. Sperm dilution experiments using alkalinized oocytes suggest the use of sperm concentrations proximate to 10(6) spermatozoa ml(-1).

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