|Minimally invasive surgical technique for egg collection from the Persian sturgeon,Acipenser persicus|
|Pourasadi, M.; Falahatkar, B.; Takami, G.A. (2009). Minimally invasive surgical technique for egg collection from the Persian sturgeon,Acipenser persicus. Aquacult. Int. 17(4): 317-321. dx.doi.org/10.1007/s10499-008-9203-9|
|In: Aquaculture International. Springer: London. ISSN 0967-6120, more|
Fish eggs; Procedures; Removal; Acipenser persicus Borodin, 1897 [WoRMS]; Iran, Caspian Sea [gazetteer]; Marine
A minimally invasive surgical technique (MIST) for the removal of ovulated eggs from Persian sturgeon Acipenser persicus was tested on broodstocks caught from the wild to determine whether it affected fecundity or hatching rates compared with the traditional stripping method of killing and removing the eggs (commonly used in hatchery). Morphological parameters of females, germinal vesicle (GV) position, weight of obtained egg, number of eggs/gram, fertilization rate, and percentage of hatching during incubation were not significantly different between the MIST and traditional stripping methods. Obtained ova were 4.8 ± 0.4 kg female−1 in the MIST and 4.6 ± 0.5 kg female−1 in traditional stripped groups, respectively; the fertilization rate was 83.4 ± 11.2% and 80.0 ± 7.2% in groups, respectively. The results of this study showed that the minimally invasive surgical technique approach is efficient, practical, and less stressful to Persian sturgeon broodstocks during artificial propagation than other reported egg collection procedures.