|DNA transfer into the Genome of Tilapia|
Ber, R.; Avtalion, R.; Timan, B.; Daniel, V. (1992). DNA transfer into the Genome of Tilapia, in: Progress in aquaculture research: proceedings of the 4th German-Isreali Status Seminar held on October 30-31, 1990. Spec. Publ. Eur. Aquacult. Soc, 17: pp. 155-165
In: (1992). Progress in aquaculture research: proceedings of the 4th German-Isreali Status Seminar held on October 30-31, 1990. Spec. Publ. Eur. Aquacult. Soc, 17. European Aquaculture Society: Oostende. ISBN 90-71625-11-7. 360 pp., more
In: Spec. Publ. Eur. Aquacult. Soc, more
|Authors|| || Top |
- Ber, R.
- Avtalion, R.
- Timan, B.
- Daniel, V.
Transfer of DNA fragments carrying the human growth hormone releasing hormone (GRH) or chloramphenicol acetyl transferase (CAT) sequences into tilapia (Oreochromis niloticus) genome and the development of 10-12 months old live transgenic fish are reported. Two approaches have been used for DNA transfer: (i) Exposure of tilapia sperm cells to DNA prior to in vitro fertilization of eggs, and (ii) Microinjection of DNA into fertilized eggs. In the first case, from among 38 tilapias developing to 25 days from eggs fertilized by sperm exposed to RSV-CAT DNA (5 x 103 copies/cell), two fish were found to be transgenic. The microinjection of a linear 2.5 kb DNA fragment containing the mouse metallothioneine-l promoter fused to the coding sequence for human GRH (1-5 x 103 copies) was carried out through the micropyle into the cytoplasm of in vitro fertilized eggs. When micro-injected at the single-cell stage the tilapia embryos exhibited a very low survival rate. Microinjection of DNA at the zygotic age of 16-64 cells allowed a 10-20% survival rate. Out of 49 fish developing from these experiments to the age of 10-12 months. seven were found to be transgenic and had integrated copies of hGRH.