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Fish intermediary metabolism and recovery after acute handling stress
Huber, M.; Dalla Via, J.; Wieser, W. (1989). Fish intermediary metabolism and recovery after acute handling stress, in: De Pauw, N. et al. (Ed.) Aquaculture: a biotechnology in progress: volume 1. pp. 875-884
In: De Pauw, N. et al. (Ed.) (1989). Aquaculture: a biotechnology in progress: volume 1. European Aquaculture Society: Bredene, Belgium. ISBN 90-71625-03-6. 1-592 pp., more

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Document type: Conference paper

Keyword
    Marine

Authors  Top 
  • Huber, M.
  • Dalla Via, J.
  • Wieser, W.

Abstract
    Handling effects on intermediary metabolism were investigated in a cyprinid fish (Rutilus rutilus). Acute locomotory stress response was induced in 4° and 20° adapted fish by mechanically inducing escaping behaviour. The treatment lasted for about 5 to 7 min until the fish were exhausted. The concentrations of the metabolites of glycolysis and of the tricarboxylic acid cycle were followed in a time series for 8h (at 4°C) and 4h (at 20°C), after the induction of stress. A high increase in concentration of glucose, glucose-1-P, glucose-6-P, fructose-6-P, pyruvate, lactate and malate was found in the stressed fish, which recovered within 8h and 4h at 4°C and 20°C, respectively. The concentration of lactate in the experiments shows a 33-fold increase, from 0.376umol.gFW-1 at routine metabolism to 12518 umol.gFW-1 under acute handling stress (at 20°C). Low temperature adapted fish (at 4°C) show only a 15-fold increase in lactate concentration under stress condition and take a longer time for recovery. It can be concluded, that handling stress influences all major pathways of intermediary carbohydrate metabolism and that not only lactate may serve as an indicator of stress in fish.

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