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Shipboard comparison of two methods of extraction and measurements of ATP applied to Antarctic water samples
Vosjan, J.H.; Nieuwland, G.; Ernst, W.; Bluszcz, T. (1987). Shipboard comparison of two methods of extraction and measurements of ATP applied to Antarctic water samples. Neth. J. Sea Res. 21(2): 107-112
In: Netherlands Journal of Sea Research. Netherlands Institute for Sea Research (NIOZ): Groningen; Den Burg. ISSN 0077-7579, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Vosjan, J.H.
  • Nieuwland, G.
  • Ernst, W.
  • Bluszcz, T.

Abstract
    During the Antarctic winter expedition (ANT V/1) of the German R.V. "Polarstern" around Elephant Island and west of the Antarctic Peninsula in May-June 1986, two research groups measured ATP in the water column by different methods: boiling in Tris buffer or extraction with NRB. These two methods gave similar results. The sample volume for the determinations, however, caused differences in the observed concentrations of ATP. Abundance and perhaps behaviour of organisms and the size of the sample will determine the upper boundary of the size of organisms in the sample. The Tris extraction values for 500 cm³ showed more scatter and slightly higher means than those of the NRB-method for 25 cm³ samples. The estimated amounts of ATP down to 3700 m depth were in the area of study, 45.1 mg·m-2 and 53.9 mg·m-2 as measured by the NRB and Tris method, respectively. Hence the biomass of small organisms in this area in May-June 1986 will have been about 11.5-13.5 gC·m-2 over a 3700 m water column.

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