|Occurrence of an ovarion opioidsystem in oviparous vertebrates: proopiomelanocortin mRNA expression in the ovary of the green water frog ,Rana esculenta|
Nabissi, M.; Polzoneti, A.M.; Carnevali, O.; Lihrmann, I.; Cartier, F.; Vaudry, H. (1995). Occurrence of an ovarion opioidsystem in oviparous vertebrates: proopiomelanocortin mRNA expression in the ovary of the green water frog ,Rana esculenta. Neth. J. Zool. 45(1-2): 163-165
In: Netherlands Journal of Zoology. E.J. Brill: Leiden. ISSN 0028-2960, more
|Authors|| || Top |
- Nabissi, M.
- Polzoneti, A.M.
- Carnevali, O.
- Lihrmann, I.
- Cartier, F.
- Vaudry, H.
The aim of the present study was to invesigate whether the proopiomelanocortin (POMC) gene is expressed in the ovary of Rana esculenta. Using a homologous probe, the presence of POMC mRNA could not be detected by Northern blot analyses in the ovary, while a positive signal was found in the pituitary gland.In contrast, after amplification of ovarion mRNA by the PCR technique, an intens signal of 480 bp was detected by Southern blotting, indicating that POMC is actually synthesized in the ovary.