In situ fluorochrome calcein marking of deep-sea molluscs using a new growth chamber
Tada, Y.; Fujikura, K.; Oguri, K.; Kitazato, H.; Tanabe, K. (2010). In situ fluorochrome calcein marking of deep-sea molluscs using a new growth chamber. Aquat. Ecol. 44(1): 217-222. http://dx.doi.org/10.1007/s10452-009-9290-3
In: Aquatic Ecology. Springer: Dordrecht; London; Boston. ISSN 1386-2588; e-ISSN 1573-5125, more
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In situ growth chamber; Fluorescent marking; Calyptogena soyoae;Calyptogena okutanii; Shell growth |
| Authors | | Top |
- Tada, Y.
- Fujikura, K.
- Oguri, K.
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| Abstract |
A new instrument designed for in situ chemical marking experiments was developed and applied to the deep-sea seep clams Calyptogena soyoae and Calyptogena okutanii. Fluorochrome calcein was used for vital staining of four living clams kept in a specially designed in situ growth chamber that was placed on the seafloor at a cold-seep site off Hatsushima Island, Sagami Bay, central Japan. The shell margins of both C. soyoae and C. okutanii were clearly stained, forming a thin fluorescent band. This method will be useful for age and growth-rate determinations of many other marine organisms with accretionary growing skeletons. |
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