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31P-NMR studies on developing eggs and larvae of plaice
Grasdalen, H.; Jørgensen, L. (1985). 31P-NMR studies on developing eggs and larvae of plaice. Comp. Biochem. Physiol. (B Biochem. Mol. Biol.) 81B: 291-294
In: Comparative Biochemistry and Physiology. Part B. Biochemistry and Molecular Biology. Pergamon: Oxford. ISSN 1096-4959, more
Peer reviewed article  

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    Embryonic development; Fish eggs; Nuclear magnetic resonance; Phosphorus compounds; Pleuronectes platessa Linnaeus, 1758 [WoRMS]; Marine

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  • Grasdalen, H.
  • Jørgensen, L.

    High-resolution 31P-NMR spectra at 40.26 MHz and 8° C are reported for intact plaice (Pleuronectes platessa) eggs at different stages of development in natural seawater. The major contributors to the NMR spectrum of eggs are inorganic phosphate, phosphatidylcholine, and phosphatidylethanolamine in a molar ratio of about 5:5:1. The relative occurrence of these compounds remained nearly constant until the first heartbeat stage, but their total amount observed by NMR, about 100 nmole/egg in unfertilized eggs, decreased by about 30%. During development of the larvae, the phospholipid resonances were gradually replaced by two broader lines at lower field, one of which was inorganic phosphate. The position of the inorganic phosphate resonance in the yolk indicated a mean internal pH of 6.2 in unfertilized eggs.

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