|The brown shrimp (Crangon crangon L.) ecdysteroid receptor complex: Cloning, structural modeling of the ligand-binding domain and functional expression in an EcR-deficient Drosophila cell line|
Verhaegen, Y.; Parmentier, K.; Swevers, L.; Rougé, P.; Soin, T.; De Coen, W.; Cooreman, K.; Smagghe, G (2010). The brown shrimp (Crangon crangon L.) ecdysteroid receptor complex: Cloning, structural modeling of the ligand-binding domain and functional expression in an EcR-deficient Drosophila cell line. Gen. Comp. Endocrinol. 168: 415-423
In: General and Comparative Endocrinology. Elsevier: New York. ISSN 0016-6480, more
Shrimp; Crangon crangon (Linnaeus, 1758) [WoRMS]; Crustacea [WoRMS]
|Authors|| || Top |
- Verhaegen, Y., more
- Parmentier, K., more
- Swevers, L.
- Rougé, P.
- Soin, T.
- De Coen, W.
- Cooreman, K., more
- Smagghe, G
cDNAs encoding ecdysteroid receptor (EcR) and retinoid X receptor (RXR) were cloned and sequenced from brown shrimp Crangon crangon (Crustacea: Decapoda), a common faunal species and commercially important in the North-West European coastal waters. A 3D model of the ligand-binding domain (LBD) of EcR was created and docking of ponasterone A (PonA) was simulated in silico. Finally, we report the transfection of expression plasmids for these receptors in the mutant Drosophila L57-3-11 cell line. Through an ecdysteroid responsive reporter assay we clearly prove the functionality of shrimp ecdysteroid receptor in the transfected L57-3-11 cell line. Our results indicate that the Drosophila L57-3-11 cell line and in silico LBD modeling can be used to study the function of crustacean ecdysteroid receptors and be applied to assess endocrine disrupting effects on non-target crustacean species.