|Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka|
|Abeysinghe, P.D.; Triest, L.; De Greef, B.; Koedam, N.; Hettiarachi, S. (1999). Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka. Hydrobiologia 413: 11-16|
|In: Hydrobiologia. Springer: Berlin. ISSN 0018-8158, more|
|Also published as |
- Abeysinghe, P.D.; Triest, L.; De Greef, B.; Koedam, N.; Hettiarachi, S. (1999). Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka, in: (2000). VLIZ Coll. Rep. 30(2000). VLIZ Collected Reprints: Marine and Coastal Research in Flanders, 30: pp. chapter 1 [Subsequent publication], more
DNA; Genetic variance; Geographical distribution; Mangrove swamps; Plant populations; Polymerase chain reaction; Population genetics; Random amplified polymorphic dna; Subpopulations; Bruguiera gymnorhiza (L.) Lamk. [WoRMS]; Bruguiera sexangula (Lour.) Poir. [WoRMS]; Marine; Brackish water
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The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random Amplified Polymorphic DNA), the genetic variation of Bruguiera populations was studied from contrasting climatic and geographic regions along the southwest coastal region of Sri Lanka. Out of 45 primers screened, 20 primers allowed us to observe polymorphism, not only between species (interspecific) but also within the species (intraspecific). Analysis of RAPD data appears to be helpful in determining the genetic relationship among populations of B. gymnorhiza and B. sexangula. RAPD markers revealed that the two species are well separated without any hybrid position between the two taxa though they occur in mixed stands. Although sampling sizes of populations of this study were small, genetic variation among B. gymnorhiza and B. sexangula populations could be observed. For B. sexangula, it was possible to differentiate each of the three populations, even when using a small number of primers.