|Distribution of proliferating cells and vasa-positive cells in the embryo of Macrostomum lignano (Rhabditophora, Platyhelminthes)|
|Willems, M.; Couvreur, M.; Boone, M.; Houthoofd, W.; Artois, T. (2010). Distribution of proliferating cells and vasa-positive cells in the embryo of Macrostomum lignano (Rhabditophora, Platyhelminthes). Belg. J. Zool. 140(Suppl.): 149-153|
|In: Belgian Journal of Zoology. Koninklijke Belgische Vereniging voor Dierkunde = Société royale zoologique de Belgique: Gent. ISSN 0777-6276, more|
Embryonic development; Macrostomum lignano Ladurner, Schärer, Salvenmoser & Rieger, 2005 [WoRMS]; Marine
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The neoblast stem cell system of flatworms is considered to be unique within the animal kingdom. How this stem cell system arises during embryonic development is intriguing. Therefore we performed bromodeoxyuridine labelling on late stage embryos of Macrostomum lignano to assess when the pattern of proliferating cells within the embryo is comparable to that of hatchlings. This pattern can be found in late embryonic stages (stage 8). We also used the freeze cracking method to perform macvasa embryonic labelling. Macvasa is a somatic and germ line stem cell marker. We showed macvasa protein distribution during the whole embryonic development. In the macvasa-positive blastomeres the protein is localized around the nucleus in the putative chromatoid bodies. However, at a specific embryonic stage, it is also ubiquitously present in the cytoplasm of some blastomeres. We compare our data with what is known from Schmidtea polychroa of the expression of the vasa-like gene SpolvlgA and the protein distribution of the chromatoid body component Spoltud-1. The embryonic origin of the somatic stem cell system and the germ line is discussed.